The members of AfricaSeeds are the countries listed below, who have been involved in its creation on 27 November 1998, or who joined since then. They are officially represented by their Ministry of Agriculture:
| 1 - Angola | 21 - Lesotho |
| 2 - Benin | 22 - Liberia |
| 3 - Burkina Faso | 23 - Madagascar |
| 4 - Burundi | 24 - Mali |
| 5 - Cameroon | 25 - Mauritania |
| 6 - Cap Vert | 26 - Mozambique |
| 7 - Chad | 27 - Namibia |
| 8 - Comoros | 28 - Niger |
| 9 - Democratic Republic of Congo | 29 - Nigeria |
| 10 - Congo Republic | 30 - Uganda |
| 11 - Côte d’Ivoire | 31 - Central African Republic |
| 12 - Eritrea | 32 - Rwanda |
| 13 - Ethiopia | 33 - Sao Tome-Principe |
| 14 - Equatorial Guinea | 34 - Senegal |
| 15 - Gabon | 35 - Sierra Leone |
| 16 - Gambia | 36 - South Africa |
| 17 - Ghana | 37 - Swaziland |
| 18 - Guinea | 38 - Tanzania |
| 19 - Guinea Bissau | 39 - Togo |
| 20 - Kenya | 40 - Zambia |
